Novel Colorimetric Assay of Polysaccharides by Alcian Blue Dye in a 96-Wel Microtiter Plate in Basidiomycete Mushroom
by Magda Semedo, Amin Karmali and Luis Fonseca
The data presented strongly support that MRL products contain high levels of beta glucans with anti-tumour activity by using a different colorimetric assay method based on alcian blue. The Alcian Blue method is based on formation of soluble complex formation between the polysaccharides and alcian blue which is specific for Beta-glucans with anti-tumour activity. (Presented at the 12th International Chemical and Biological Engineering Conference (CHEMPOR 2014), organized by the Department of Chemical Engineering of the Faculty of Engineering - University of Porto and Ordem dos Engenheiros (the Portuguese Engineers Association), held in Porto, Portugal, between the 10th and 12th of September, 2014.